Extended Data Fig. 1: Transient nature of azide-bearing motifs installed through metabolic glycoengineering.
From: Engineered nascent living human tissues with unit programmability
a, Fluorescence microscopy of negative control and azide-bearing hASCs at 0 and 7 days post-glycoengineering. Blue channel, cell nuclei; green channel, cell-surface azides. Scale bars, 100 µm. b, Flow cytometry time-lapse evolution of azide-bearing motifs in hASCs cultured in standard medium (that is, absent of Ac4ManNAz). Normalized mean fluorescence intensity (MFI) is expressed following the correction of non-specific background from negative controls (that is, subtraction of MFI from native hASCs treated with DBCO-PEG4-Rhod110. Data represented as mean ± s.d., n = 4 replicates. c, Representative flow cytometry histogram plots of time lapse evolution of glycoengineered hASCs versus negative control. d, Micrograph of glycoengineered hASCs 7 days post-functionalization combined with the cell-tethering component (HA-DBCO) and unable to form Cellgel constructs. Scale bars, 5 mm.
