Extended Data Fig. 2: Characterization of focal adhesions and the role of adhesive ligands in cell tumbling.
From: Cell tumbling enhances stem cell differentiation in hydrogels via nuclear mechanotransduction
(a) Representative immunofluorescence images for focal adhesion markers in SG (top) and CG (bottom) after 24 hours of chondrogenic induction and Fibronectin after 12 hours of chondrogenic induction as observed from 3 independent experiments. Marker of interest- green, F-Actin- red, and DAPI- blue. Scale- 10μm. (b) Centroid speed and (c) Major axis angular speed upon perturbation of adhesive ligands. N ≥ 15 across 3 gels per condition (d) Nuclear movement speed upon perturbation of adhesive ligands. N ≥ 20 across 3 gels per condition. ns, not significant; *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001. P value, one–way ANOVA with Tukey’s multiple comparisons test.
