Extended Data Fig. 3: Characterization of Ezrin in cell tumbling.
From: Cell tumbling enhances stem cell differentiation in hydrogels via nuclear mechanotransduction
Note- NSC is the Ezrin inhibitor NSC668394. (a) Representative western blotting images and (b) Quantifications of western blotting images after 24 hours of chondrogenic induction. Protein expression for each marker was normalized to GAPDH. N = 3 biological replicates per condition. Data reported represent mean value ± s.d. (c) Representative immunofluorescence images for Ezrin (green), F-Actin (red) and DAPI (blue) in SG (top), SG with ezrin inhibitor (middle), and CG (bottom) after 24 hours of chondrogenic induction. Scale- 10μm. (d) Quantification of interquartile range of Ezrin intensity at the cell cortex highlighting ezrin expression heterogeneity. N ≥ 15 across 3 gels per condition (e) Cortical Actin, (f) Centroid speed, (g) Major axis angular speed, and (h) nuclear movement speed upon Ezrin inhibition. N ≥ 15 across 3 gels per condition ns, not significant; *p < 0.05, **p < 0.005, ***p < 0.001, ****p < 0.0001. P value, one–way ANOVA with Tukey’s multiple comparisons test.
