Extended Data Fig. 10: Force transmission and collective deformation provide a competitive advantage.
From: Force transmission is a master regulator of mechanical cell competition
(a) Comparison of the normalized stress susceptibility corresponding to Fig. 4h. Random positions of E-cad KO cells at the interface, E-cad KO and WT before (t = −40 and t = −20 in Fig. 5h) and after the extrusion event (t = 0 and t = 20) are shown. n = 12 (KO, WT) and n = 20 (Random) time intervals from N = 2 independent experiments. (b) Exemplary airyscan image of contractile actomyosin cables at the interface. Actin in white, phospho-myosin in red. Actomyosin fibers are interrupted between E-cad KO cells (white arrow) but can span multiple cells between WT cells (magenta arrow). (c) Phase contrast images of WT and E-cad KO cells colliding. Magenta outlines show manual area quantification of WT cells. (d) Manual segmentation of the WT apical area following tissue collision. Each datapoint represents one cell. n = 31 (before) to n = 54 (15 h) cell, as they migrate into the FOV or divide. Data from N = 1 preparation. (e) Quantification of the extrusion rate over time. T = 0 indicates the timepoint of collision. n = 4 movies from N = 2 independent experiments (f) Example of WT cell doublet being eliminated (blue circles) by surrounding E-cad KO cells. P-values from Kruskal-Wallis test corrected for multiple comparisons (Dunn’s test). Means and standard deviations are shown. Values normalized to maximal value. Scale bar 50 µm (c), 20 µm (f), 10 µm (b).
