Extended Data Fig. 1: Modulation of intercellular adhesion in MDCK and MCF10A cells.
From: Force transmission is a master regulator of mechanical cell competition
(a) Increased alpha-catenin intensity at junctions between E-cad-overexpressing (E-cad OE) cells (described in Adams et al., JCB, 1998) compared to WT cells. Upper: representative confocal image. Lower: Junctional alpha-catenin intensity normalized to WT cells. (b) Representative images of mixed culture of MDCK WT lifeAct-mCherry and E-cad OE cells. Yellow outlines show initial clusters, red circles mark extrusions. (c) Area development of MDCK WT lifeAct-mCherry cells in competition with E-cad OE cells. n = 10 movies from N = 2 independent experiments (d) Extrusion rates of WT lifeAct-mCherry and E-cad OE cells. Each datapoint shows one time interval from n = 4 movies and N = 2 independent experiments. P-value from unpaired, two-sided t-test. (e) Mixed culture and (f) collision of MCF10-A EGFP (green) and MCF10-A E-cad KO (not fluorescent) cells. Magenta outlines show initial clusters. Red circles mark extrusions. Below: Area development of MCF10A E-cad KO cells compared to MDCK E-cad KO cells (shown in Fig. 1) normalized to initial value. MCF10A data: n = 5 movies from N = 2 independent experiments for coculture and collision. Data are presented as mean values +/− SD. Scale bars 100 µm (C,D); 50 µm (b); 10 µm (a).
