Extended Data Fig. 2: Different validations of stress measurements.
From: Force transmission is a master regulator of mechanical cell competition
(a) Isotropic stresses before and after collision of E-cad KO (green) and WT tissues. Before collision, tension is highest at the migration front of both cell types. After collision, the WT cells are under compression. (b) Example confocal image of mixed culture (LifeAct-E-cad KO in green) stained for cell boundaries (ZO-1, magenta). (c) Example image and average relative pressure inferred based on cell shape, as described in Kong et al., Sci. Rep. 2019. Each datapoint represents a single cell. n = 1000 cells representing N = 2 independent experiments. (d) Representative images of WT cells expressing CAAX-GFP mixed with E-cad KO cells expressing lifeAct-GFP before and after laser ablation. (e) Comparison of the recoil velocity measured after the ablation. Each datapoint represents one ablation representing n = 15 (WT) and n = 13 (E-cad KO) movies from N = 2 independent experiments. P-values from unpaired, two-sided t-test. Scale bars 100 µm (a,b); 10 µm (d).
