Extended Data Fig. 5: Production efficiency of intracellular and extracellular ROS and their influence on NSCs.
(a) Measurement of ROS levels in NSCs after incubation with BTO nanoparticles and treatment with ultrasound at different powers and (b) associated analysis of the ROS level data. (c) ROS levels in NSCs after incubation with BTO/rGO nanostickers and treatment with ultrasound at different powers and (d) associated analysis of the ROS level data. (e) Images from the live/dead assay and (f) analysis of the survival rate of NSCs after incubation with BTO nanoparticles and treatment with ultrasound at different powers (green: live cells; red: dead cells). (g) Images from the live/dead assay and (h) analysis of the survival rate of NSCs after incubation with BTO/rGO nanostickers and treatment with ultrasound at different powers (green: live cells; red: dead cells). (i–j) mRNA levels of Tuj1 and MAP2 in NSCs that were incubated with BTO nanoparticles and treated with ultrasound at different powers. (k–l) mRNA levels of Tuj1 and MAP2 in NSCs that were incubated with BTO/rGO nanostickers and treated with ultrasound at different powers. The data in b, d, f, and h-l are shown as mean ± s.d., n = 3 independent biological samples. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparisons tests (NS: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001).
