Extended Data Fig. 8: BTO/rGO piezoelectric nanostickers promote the neuronal differentiation of NSCs in vivo.
(a) Schematic of the in vivo experiments. (b) Representative image of surgery for NSC injection. (c) Representative image of the brain after 7 days of treatment. (d–e) Representative images of immunofluorescence images staining Tuj1/GFAP and MAP2/GFAP in brain sections from one of three rats in each group on day 7 (red: GFAP; green: MAP2 or Tuj1; blue: DAPI). (f) mRNA levels of Tuj1 and MAP2 in the four groups. (g) Western blot of Tuj1 and MAP2 levels in the four groups and (h) associated analyses. The data in f and h are shown as mean ± s.d., n = 3 independent biological samples. Statistical analyses were performed via one-way ANOVA with Dunnett’s multiple comparisons tests (NS: not significant, *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001). Figure elements in a have been created with Figdraw.com.
