Extended Data Fig. 3: HIV-2/SIVmac Vpx suppresses NF-κB regulated genes in HEK293T cells and ISD- stimulated innate immune responses in EA.hy926 cells. | Nature Microbiology

Extended Data Fig. 3: HIV-2/SIVmac Vpx suppresses NF-κB regulated genes in HEK293T cells and ISD- stimulated innate immune responses in EA.hy926 cells.

From: HIV-2/SIV Vpx targets a novel functional domain of STING to selectively inhibit cGAS–STING-mediated NF-κB signalling

Extended Data Fig. 3

Aliquots of the samples used in Fig. 1a–c were analyzed by immunoblotting using anti-Flag and anti-HA antibody (A,C,E) and RT-qPCR (B,D,F) (n = 3 independent biological experiments). Means and standard deviations are presented. The statistical significance analyses were performed using a two-sided unpaired t-test. G, Detection of HIV-2/SIVmac HA- Vpx and endogenous SAMHD1 in HIV-2/SIVmac Vpx- transduced EA.hy926 cells. (One representative immunoblotting result out of n = 2 independent biological experiments is shown.) H, Transduced EA.hy926 cells were transfected with ISD (2 μg/mL) for 12h. Total RNA was prepared and analyzed for the transcriptional level of the indicated genes by RT-qPCR (n = 3 independent biological experiments). Means and standard deviations are presented. The statistical significance analyses were performed using a two-sided unpaired t-test.

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