Extended Data Fig. 4: Saturation of TRIM5α-mediated restriction in primary human macrophages.
From: Cyclophilin A protects HIV-1 from restriction by human TRIM5α
Luc or TRIM5 knockdown macrophages were simultaneously challenged with a constant amount of single-cycle, VSV G-pseudotyped HIV-1NL4-3GFP containing CA-P90A and the indicated quantities of HIV- 1NL4-3 VLPs harboring either WT CA or CA-P90A. The percentage of GFP+ cells was assessed by flow cytometry at day 3 post-challenge. Data shown here are representative of four independent experiments performed on cells from four blood donors.
