Extended Data Fig. 4: Stronger immune response observed during basolateral infection of hIECs is not due to differences in transcript stability. | Nature Microbiology

Extended Data Fig. 4: Stronger immune response observed during basolateral infection of hIECs is not due to differences in transcript stability.

From: Asymmetric distribution of TLR3 leads to a polarized immune response in human intestinal epithelial cells

Extended Data Fig. 4

(a) Polarized T84 cells were infected apically or basolaterally with MRV. 16 hpi cells were treated with actinomycin D to block transcription. RNA samples were collected at the indicated times post-actinomycin treatment. (b) Relative amounts of type I (upper panel) and type III IFN (lower panel) transcripts (normalized to the time of actinomycin treatment) were evaluated by q-RT-PCR (left panel). The half-life of each transcripts was determined (right panel). Results indicate the mean +/- sd. n=3. P values were determined from a two-tailed unpaired t test using GraphPad Prizm.

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