Extended Data Fig. 5: Basolateral infection of T84 cells by MRV leads to the upregulation of many immune related genes.

Polarized T84 cells were infected apically or basolaterally with MRV. 16 hpi, RNA was collected and subjected to microarray analysis. Expression values were quantile-normalized after background correction and log2-transformed. Unspecific filtering was performed by discarding genes with non- significant p-values in any sample. (a) Differentially expressed genes between apical infection and mock infected cells as well as between basolateral infection and mock infected cells were identified using the empirical Bayes approach and are shown in a Venn diagram. (b) Heatmap of expression values of significantly upregulated genes in apically and basolaterally infected cells were scaled across samples and hierarchical clustering was performed using euclidean distance and complete linkage. Gene values and their reference numbers can be found in the Supplementary Dataset. (c) KEGG analysis of significantly upregulated genes upon basolateral infection of T84 cells.