Extended Data Fig. 9: TLR3 and AP-1B are not found together with immunofluorescence staining. | Nature Microbiology

Extended Data Fig. 9: TLR3 and AP-1B are not found together with immunofluorescence staining.

From: Asymmetric distribution of TLR3 leads to a polarized immune response in human intestinal epithelial cells

Extended Data Fig. 9

(a) TLR3 antibody specificity was controlled by transfecting HEK 293T (naturally non-TLR3 expressing) cells with a plasmid expressing TLR3. 48 h post-transfection cells were fixed and stained for TLR3 expression (red). Nuclei were stained with DAPI (blue). Representative images are shown. (b) T84 cells were seeded as spheroids. Five days post-seeding spheroids were fixed and stained for Laminin (red), and either TLR3 (green) or AP-1 (green). Nuclei were stained with DAPI (blue). Representative image is shown. Scale bar represents 20μm. (c) Same as b, except TLR3 and AP-1 were co-stained. Laminin (red), TLR3 (green), AP-1 (magenta), and nuclei were stained with DAPI (blue). Two representative images are shown. Scale bar represents 20μm in full size image and 5μm in zoom in.

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