Extended Data Fig. 3: Side specific induction of IFN production upon viral infection in the human intestinal epithelial lines. | Nature Microbiology

Extended Data Fig. 3: Side specific induction of IFN production upon viral infection in the human intestinal epithelial lines.

From: Asymmetric distribution of TLR3 leads to a polarized immune response in human intestinal epithelial cells

Extended Data Fig. 3

(a-b) SKCO15 cells were seeded on transwells. Following establishment of barrier function, cells were infected apically or basolaterally with MRV. (a) 16 hpi, the production of type I (IFNβ) and type III (IFNλ2/3) IFNs was assessed by q-RT-PCR. Results are normalized to mock infected samples and show the mean +/- sd. n=3. (b) Efficiency of the primary MRV infection in SKCO15 cells was controlled by immunofluorescence against the non-structural protein μNS (red). Cell nuclei were stained using DAPI (blue). Three biological replicates were performed. Representative images are shown. Scale bar =100 μm. (c) T84 cells were infected apically or basolaterally with MRV. RNA samples were collected at the indicated times post-infection and production of type I (IFNβ) and type III (IFNλ2/3) interferons was measured by q-RT-PCR. Results are normalized to mock infected samples and show the mean +/- sd. n=3. (d) T84 cells were infected apically or basolaterally with MRV. Supernatants were collected at the indicated times post- infection and analysed by ELISA for the secretion of type III (IFNλ2/3) interferon. Results show the mean +/- sd. n=3. Black line indicates the limit of detection. (a-d) A=apical infection. B =basolateral infection. (a,d) P values were calculated using a two-tailed unpaired t test in GraphPad Prizm.

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