Extended data 3: Characterization of the ΔhemBΔfumC double mutant.
(A) Growth of the ΔhemBΔfumC double mutant as compared to WT LAC, the ΔhemB or ΔfumC single mutants on BHI and blood agar plates. Various dilutions of bacterial cultures were plated. Image shown is from 1 representative experiment, n = 3 independent experiments. (B) Growth of the ΔhemBΔfumC double mutant as compared to WT LAC and the ΔhemB single mutant in liquid LB culture. n = 3 independent experiments. (C) Cytokine measurements from the skin of mice 5 days post infection with WT LAC or the ΔhemBΔfumC mutant. Data represent mean ± SEM, n = 4 independent animal samples.. Statistical analysis was performed by one-way ANOVA, ****p < 0.0001, ***p < 0.001, **p < 0.01, *p < 0.05.
