Extended Data Fig. 2: Body weight changes and histopathological findings in infected Syrian hamsters.
a, Syrian hamsters were intranasally inoculated with 106 PFU of WT-KK001-I38 (H1N1pdm), MUT-KK001-I38T (H1N1pdm), WT-KK003-I38 (H1N1pdm), or MUT-KK003-I38T (H1N1pdm), or MEM containing 0.3% BSA (mock). Body weights of virus-infected (n=3) and mock-infected animals (n=4) were monitored daily for 6 days. Data are presented as the mean percentages of the starting weight ± SD. P-values were calculated by using pairwise comparisons after a linear mixed model analysis (*P < 0.05; **P < 0.01). Asterisks next to data points depict statistically significant differences between mutant and wild-type viruses. See Methods for more details regarding the statistical analysis. b, Representative pathological images of lungs infected with WT-KK001-I38 (H1N1pdm, n=2), MUT-KK001-I38T (H1N1pdm, n=2), WT-KK003-I38 (H1N1pdm, n=2), MUT-KK003-I38T (H1N1pdm, n=2), WT- KK015-I38 (H3N2, n=2), MUT-KK015-I38T (H3N2, n=2), WT-GR117-I38 (H3N2, n=2), or MUT-GR117-I38T (H3N2, n=2) on Day 6 post-infection. Left panels, hematoxylin and eosin (HE) staining. Scale bars, 200 μm. Right panels, immunohistochemistry (IHC) for influenza viral antigen detection. Scale bars, 100 μm.
