Extended Data Fig. 4: Genomic context and conservation of camA. | Nature Microbiology

Extended Data Fig. 4: Genomic context and conservation of camA.

From: Epigenomic characterization of Clostridioides difficile finds a conserved DNA methyltransferase that mediates sporulation and pathogenesis

Extended Data Fig. 4

(a) CamA protein alignment among Clostridiales (C. mangenotti LM2 (587 aa, 56% identity), C. sordellii (598 aa, 53% identity), C. bifermentans WYM (579 aa, 53% identity), C. dakarense sp. nov (580 aa, 63% identity), Peptostreptococcaceae bacterium VA2) and Fusobacteriales (Psychrilyobacter atlanticus DSM 19335) using ClustalX. The nine conserved motifs (I-VIII and X) typically found in MTases are highlighted. (b) Phylogenetic tree obtained from the MTase alignment. (c) Phylogenetic tree of the 36 C. difficile strains colored by clade (hypervirulent, human/animal (HA) associated) and MLST sequence type (ST). Shown is the genomic context of camA across the entire dataset. (d) Expanded view of the region shown in Fig. 1f. The example shown (including coordinates) refers to the reference genome of C. difficile 630. + and – signs correspond to the sense and antisense strands respectively. Vertical bars correspond to the distribution of the CAAAAA motif.

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