Extended Data Fig. 7: Mice provoke efficient clearance and strong resistance against O-Ag-deficient Shigella strain.
a, Macroscopic images of ShigellaM90T or ShigellaΔgalU infected livers after 6 or 24 h p.i. b, Liver sections of mice infected for 24 h with ShigellaM90T or ShigellaΔgalU. Sections were stained against Shigella and DAPI. Scale bar, 2 mm. Detail scale bar, 500 µm. c, Confocal images of liver sections of ShigellaM90T or ShigellaΔgalU infected mice after 24 h p.i. Liver sections were stained with TUNEL, Shigella antibody and DAPI. Scale bar overview, 100 µm. Scale bar detail, 20 µm. d, Western blot analysis of whole cell lysates of BMDMs infected with ShigellaM90T or ShigellaΔgalU (MOI: 10, 3 h) stained for caspase-3 and -7. Tubulin served as a loading control. e, Western blot analysis of whole cell lysates of BMDM, liver or colon of caspase-3WT/WT and caspase-3-/- mice stained for caspase-3 and -7. Tubulin served as a loading control. f, Weight loss in percentage was calculated before infection and 48 h p.i. of caspase-3WT/WT and caspase-3-/- mice infected with ShigellaΔgalU. Statistical differences were analysed by two-tailed unpaired student’s t-test (n = 7 (Caspase-3WT/WT) and 9 (Caspase3-/-)). g, Histological analysis of livers from ShigellaΔgalU infected caspase-3WT/WT and caspase-3-/- mice. Sections were stained by H&E. Scale bar, 100 µm. All experiments are representatives of at least two independent experiments and are presented as mean ± s.d. * p ≤ 0.05. Weights in Western blots are in kDa.
