Extended Data Fig. 8: Mice provoke efficient clearance and strong resistance against O-Ag-deficient Shigella strain.
a, Liver sections of ShigellaΔgalU infected caspase-3WT/WT and caspase-3-/- mice after 48 h p.i. Liver sections were stained with TUNEL, Shigella antibody and DAPI. Scale bar overview, 100 µm. Scale bar detail, 20 µm. b, TUNEL positive Shigella areas were counted in livers. 3-10 Shigella areas of each mouse were counted for each genotype and percentage of TUNEL positive areas was determined. Statistical differences were analysed by two-tailed unpaired student’s t-test (n = 40 (Caspase-3WT/WT) and 33 (Caspase3-/-) Shigella positive areas of 4 different mice). c, ShigellaM90T or ShigellaΔgalU were treated with complement preserved human or murine serum for 0, 2 and 4 h. OD600 (left panel) or CFU (right panel) was analysed (n = 3 technical replicates). Statistical significance to the corresponding untreated value was determined by one-way ANOVA followed by Sidak’s post-analysis. All others are ns (OD600: P = 0.5656; > 0.9999 for ShigellaM90T and ShigellaΔgalU to murine serum, respectively and CFU: P = 0.8806; 0.3889 for ShigellaM90T and ShigellaΔgalU to murine serum, respectively and ShigellaM90T to human serum P = 0.2996). All experiments are representatives of at least two independent experiments and are presented as mean ± s.d. **** p ≤ 0.0001; *** p ≤ 0.001; ** p ≤ 0.01.
