Extended Data Fig. 3: Cytosolic appearance of Gram-negative bacteria determines its capability to inhibit effector caspases.
a, CFU analysis of indicated intracellular bacteria. Statistical significance to the ShigellaM90T infected value was determined by one-way ANOVA followed by Sidak’s post-analysis (n = 3 biologically independent samples). b-c, Cytosolic extracts of HeLa cells infected with the indicated bacterial strains were activated with 100 ng ml−1 Trail or 4 µM STS and caspase-3/-7 activity was analysed. Statistical significance to the ShigellaM90T infected value was determined by one-way ANOVA followed by Sidak’s post-analysis (n = 3 technical replicates of independent staining). d, Live cell imaging of HeLa cells infected with the indicated pathogens and treated with 100 ng ml−1 Trail. Scale bar, 100 µm. e, HCT cells were infected with ShigellaM90T, Salmonella or SalmonellaΔSifA. Cytosolic extracts of cells (MOI: 30, 3 h, 100 µg) were incubated with recombinant active caspase-8 and caspase-3/-7 enzymatic activity were analysed. Statistical significance to the corresponding non-infected infected value was determined by one-way ANOVA followed by Sidak’s post-analysis (n = 3 technical replicates of independent staining). All experiments are representatives of at least two independent experiments and are presented as the mean ± s.d. **** p ≤ 0.0001; *** p ≤ 0.001; ** p ≤ 0.01; * p ≤ 0.05.
