Fig. 1: Sequencing of microbial mock community (HM-277D) with Illumina and MinION sequencing.

a, Correlation plot comparing the expected proportions of DNA from each of the 20 species in the mock community with the proportions of sequence obtained by MinION (left, log-transformed Pearson’s r = 0.94) and Illumina platforms (log-transformed Pearson’s r = 0.97). The right-hand plot shows the correlation between MinION and Illumina (log-transformed Pearson’s r = 0.98). The grey region either side of the fit line represents the 95% CIs. b, MEGAN³⁷ taxonomy tree representing taxa assigned from the mock community as sequenced by Illumina MiSeq (green) and ONT MinION (brown). The height of the bars indicates the relative number of reads assigned, with normalized counts given next to the taxa name. Asterisks represent species assigned by MEGAN but not specified as members of the mock community. Numbers assigned to taxa correspond to Illumina MiSeq (left) and ONT MinION (right) normalized counts, respectively.