Extended Data Fig. 8: The effect of oxygen on the activity of the nifH promoters.
From: Control of nitrogen fixation in bacteria that associate with cereals
Expression from the nifH promoters was analysed in E. coli MG1655 containing the controller plasmid pMR104, P. protegens Pf-5 MR10 (for K. oxytoca) and MR9 (for P. stutzeri and A. vinelandii) at varying initial oxygen levels in the headspace. The three nifH promoters were induced with 0.05 mM IPTG and 0.5 mM IPTG in E. coli MG1655 and P. protegens Pf-5, respectively, and incubated at varying initial oxygen concentrations. Error bars represent standard deviation from three independent experiments on different days.
