Extended Data Fig. 1: Nitrogenase activity in wild-type R. sp. IRBG74 and a Δnif mutant strain, in which the native nif clusters are deleted. | Nature Microbiology

Extended Data Fig. 1: Nitrogenase activity in wild-type R. sp. IRBG74 and a Δnif mutant strain, in which the native nif clusters are deleted.

From: Control of nitrogen fixation in bacteria that associate with cereals

Extended Data Fig. 1

(a) The nif clusters in R. sp. IRBG74. The deleted regions generated by the suicide plasmids pMR45–46 are marked (Methods). (b) Transfer of native nif constructs into R. sp. IRBG74. Nitrogenase activity was detected only from the transfer of the R. sphaeroides nif cluster into R. sp. IRBG74 MR17 (ΔhsdR, recA) but not into R. sp. IRBG74 MR19 (ΔhsdR, recA Δnif). Expression of A. caulinodans nifV on the plasmid pMR49 in R. sp. IRBG74 MR17 was induced by 0.5 mM IPTG. The co-transfer of the complete A. caulinodans nif cluster on the two plasmids pMR19 and pMR20 did not yield activity in R. sp. IRBG74 MR17. Error bars represent standard deviation from three independent experiments on different days. Asterisk indicate ethylene production below the detection limit. Rsp, R. sphaeroides; Aca, A. caulinodans. (c) Plasmid maps used in (b).

Back to article page