Extended Data Fig. 2: Bacterial load and composition in feces of non-antibiotic treated, antibiotic-treated and commensal consortia inoculated mice.

Experimental mouse groups are indicated on the left-hand side. From top to bottom, “–ABX” are mice before experimental manipulations; “+ABX” are mice administered MNVA antibiotics in drinking water; “+Microbiota” are mice that have been administered MNVA antibiotics in drinking water and then transferred intestinal and upper airway microbiota; the remaining groups (for example “+Bacteroidetes”) are mice administered MNVA antibiotics in drinking water and then orally administered the indicated commensal consortium (as in Fig. 2). For all of these groups, from left to right, the data are: total 16s rRNA gene copies in adult mouse feces; relative abundance of bacterial taxa in mouse feces (each bar represents the means of n=5 animals); and 16s rRNA gene copies in adult mouse feces of indicated commensal consortia (for example, in the mice administered the “+Bacteroidetes”, “Consortia colonization” levels are the 16s rRNA gene copies for Bacteroidetes in those mice). Statistical comparisons were by Kruskal–Wallis test with Dunn’s multiple comparison test, vertical lines indicate median values.