Extended Data Fig. 10: Removal of the lipid carrier from nascent peptidoglycan inhibits SagB-SpdC cleavage.
a, Schematic of experiment shown in b. b, Radiolabeled peptidoglycan oligomers (lane 3) were treated with Colicin M (lane 4), which removes the lipid carrier, leaving a pyrophosphate on the reducing terminus of the glycan. Lipid-linked and de-lipidated oligomers were then treated with SagB-SpdC (lanes 5 and 6, respectively). Comparison of lane 3 to lane 5 shows changes to the sample characteristic of SagB-SpdC activity, whereas comparison of lane 4 to lane 6 shows no change, indicating that SagB-SpdC was not able to cleave oligomers lacking the lipid carrier.
