Extended Data Fig. 3: Binding parameters of SmbA protein in complex with nucleotides.

(a-j) Isotherms representing binding of recombinant SmbA wild type or mutant proteins with c-di-GMP (a-d), ppGpp (e-h), pppGpp (i) and GMP (j) as measured by ITC. Please see Supplementary Table 2 for detailed experimental conditions and measured parameters.