Extended Data Fig. 8: SmbA regulates Caulobacter surface attachment.
(a) Fraction of Caulobacter wild type and smbA mutants with visible holdfast. (b) Representative microscopy images (overlay of phase contrast and fluorescent GFP channels) of cultures with stained holdfast (indicated by red arrows) used for calculations described above. For each strain, similar results were obtained from three independent cultures. (c) Transcription of hfiA in Caulobacter wild type and mutant strains as indicated. A lacZ transcriptional reporter was used to determine hfiA promoter strength. β-galactosidase activities were determined in strains containing different smbA alleles on the chromosome. A staR deletion mutant was used as a control. (d) Attachment of hfiA deletion strains harbouring different smbA alleles as measured by crystal violet stain. In panels (a, c-d), data represent the mean of n = 3 biological replicates ± SD.
