Extended Data Fig. 4: Biogeochemical measurements of in situ soil gas concentrations and validation of static chamber setup.
From: Trace gas oxidizers are widespread and active members of soil microbial communities
(a) Mean soil-gas profiles normalized to the respective ambient air concentration (dashed line) of four biological replicates collected at depths of 0, 2, 4, 6, 8, 10, and 16 cm across four Australian ecosystems (n = 112). Note that the different gases were sampled at identical depths, but points are plotted slightly offset on the y-axis for better visibility of error bars. (b) Laboratory static chamber incubations (n = 3) to control for abiotic release of trace gases from chamber’s plastic components. Chamber headspace mixing ratios of hydrogen (H2), carbon monoxide (CO), and methane (CH4) were measured at eight time points during ~25 minutes of incubation time of an inert stainless steel surface. Measurement setup, sampling procedure, and sampling frequency followed those applied during the in situ chamber incubations performed to measured soil-atmosphere flux of trace gases. Circles indicate mixing ratio averaged from three independent incubations, with the vertical lines denoting one standard deviation. Dashed lines indicate the mixing ratio of trace gas averaged across three air samples.
