Extended Data Fig. 6: Interaction network between kefir species based on milk acidification assay.
From: Metabolic cooperation and spatiotemporal niche partitioning in a kefir microbial community
a, Schematic depiction of the method used to map metabolic interactions based on fermentation acidification kinetics. Species were grown in 96-well plates alone or in pairs; acidification of milk was assessed with a soluble pH-indicator. Positive interactions were identified as those that showed increased acidification in co-culture compared to mono-cultures, while negative interactions as those that showed decreased acidification in co-culture. b, Network of metabolic interactions between kefir species (Interaction calling based on N = 6; 3 biological and 2 technical replicates). Node sizes indicate number of interactions. Raw R-values extracted from scan images are provided in Supplementary Table 24.
