Extended Data Fig. 5: SprF1 is a non-coding RNA. | Nature Microbiology

Extended Data Fig. 5: SprF1 is a non-coding RNA.

From: RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus

Extended Data Fig. 5

a, Growth kinetics of S. aureus N315 strains carrying pALC, pALCΩsprF1-Flag, or pALCΩsprG1-Flag in presence (right panel) or absence (left panel) of 1 µM anhydrotetracyline (aTc). Error bars show the mean and standard deviation of three independent experiments (n = 3). b, Northern blot analysis of SprF1- and SprG1-flagged RNAs expression levels in S. aureus N315 strains carrying pALC, pALCΩsprF1-Flag, or pALCΩsprG1-Flag after induction by 1 µM anhydrotetracyline (aTc). 5 S rRNA is the internal loading control (n = 3). Source data are provided as a Source Data file.

Source data

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