Extended Data Fig. 8: Toeprint assays of SprF1 antitoxin. | Nature Microbiology

Extended Data Fig. 8: Toeprint assays of SprF1 antitoxin.

From: RNA antitoxin SprF1 binds ribosomes to attenuate translation and promote persister cell formation in Staphylococcus aureus

Extended Data Fig. 8

a, Toeprint assays of 0.25 pmol alsS mRNA or SprF1 in the absence (-) or presence (+) of 2 pmol of purified E. coli 70 S ribosomes and 12.5 pmol of uncharged tRNAfMet. The A, C, G and U lanes represent the alsS mRNA or SprF1 sequencing lanes. The arrows represent the toeprint position when observed. The gel illustrates one representative experiment among three independent experiments (n = 3). b, Toeprint assays of 0.25 pmol SprF1 or SprF1∆6 mutant in the absence (-) or presence (+) of 2 pmol of purified E. coli 70 S ribosomes and 12.5 pmol of uncharged tRNAfMet. The A, C, G and U lanes represent the SprF1 sequencing lanes. The arrow represents the toeprint position when observed. The gel illustrates one representative experiment among three independent experiments (n = 3). Source data are provided as a Source Data file.

Source data

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