Extended Data Fig. 5: Phage resistance in strains igaA and mdoG is dependent on the Rcs pathway. | Nature Microbiology

Extended Data Fig. 5: Phage resistance in strains igaA and mdoG is dependent on the Rcs pathway.

From: The Rcs stress response inversely controls surface and CRISPR–Cas adaptive immunity to discriminate plasmids and phages

Extended Data Fig. 5

a, Deletion of rcsB restores susceptibility of strains igaA and mdoG to flagellatropic phage OT8. Deletion of rcsA partially sensitizes igaA to OT8, while mdoG remains largely resistant (n = 3 biologically independent samples). MOI, multiplicity of infection. These results support the partial role for rcsA in swimming motility (Extended Data Fig. 4) and flagella gene repression (Extended Data Fig. 6) observed in igaA but not mdoG. b, Deletion of either rcsB or rcsA restores susceptibility of strain igaA to phage LC53 resistant (n = 3 biologically independent samples). Lines in a and b represent the mean +/− the standard deviation.

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