Extended Data Fig. 6: Genetic organization and expression levels of Rcs-affected loci.
a, Expression of the flhDC operon (flagella master regulator) promoter (PflhDC) is reduced in Rcs-activated strains. In strain igaA, restoration of expression to WT levels occurs upon deletion rcsB and rcsD, while rcsA deletion partially restores expression. Repression of PflhDC is independent of rprA. In mdoG, flhDC expression is increased upon deletion of rcsB and rcsD but is unaffected by rcsA deletion (n = 5 biologically independent samples). MFI, median fluorescence intensity. b, Expression of the ompW promoter (PompW) is reduced in Rcs-activated strains. In strain igaA, an increase in expression is observed upon deletion rcsB, rcsD, or rcsA. In mdoG, the slight decrease in ompW expression does not alter phage LC53 infectivity in liquid but may contribute to an observed reduction in plaque formation on solid media (Extended Data Fig. 4). Repression of ompW is independent of rprA in the igaA mutant (n = 5 biologically independent samples). All bars indicate the mean and error bars represent the s.e.m. Two-sided t-tests were used to determine statistical significance. Detailed statistical testing can be found in the accompanying Source Data file. ****P < 0.0001; **P < 0.01; ns, not significant.
