Extended Data Fig. 3: The Rcs pathway mediated changes in type I expression and interference.
a, Repression of type I-E cas3 expression requires rcsB in both strains mdoG and igaA, while rcsA contributes to repression only in strain igaA. The reduction of cas3 expression is independent of rprA (n = 5 biologically independent samples). MFI, median fluorescence intensity. b, Repression of type I-F cas1 expression requires both rcsB and rcsA in strain igaA and is independent of rprA (n = 5 biologically independent samples). c, CRISPR-Cas interference measured through conjugation efficiency of plasmids carrying protospacers matching endogenous CRISPR arrays (targeted) or control plasmids (untargeted). Type I-E CRISPR-Cas interference of the igaA strain is restored upon deletion of rcsB, and partially restored upon deletion of rcsA (I-E targeted). Type I-F interference levels of the igaA strain are restored upon deletion of rcsB or rcsA (I-F targeted). Conjugation efficiency of untargeted plasmids is similar amongst rcsA or rcsB deletions and isogenic parental strains (untargeted) (n = 3 biologically independent samples). All bars shown are the mean and error bars represent the s.e.m. For panels a and b, two-sided t-tests were used to determine statistical significance. Detailed statistical testing can be found in the accompanying Source Data file. ****P < 0.0001; **P < 0.01; ns, not significant.
