Extended Data Fig. 8: The gating strategy and the frequency of circulating Tfh cell subsets in recovered COVID-19 individuals. | Nature Microbiology

Extended Data Fig. 8: The gating strategy and the frequency of circulating Tfh cell subsets in recovered COVID-19 individuals.

From: Spike-specific circulating T follicular helper cell and cross-neutralizing antibody responses in COVID-19-convalescent individuals

Extended Data Fig. 8

a, Representative gating strategy for circulating Tfh (PD-1+CXCR5+ CD4+ T) cells, CXCR3+ and CXCR3− Tfh cells. Gating of cell populations is based on the mean fluorescence intensity ‘minus one’ (FMO). b–e, Comparison of between healthy controls (HC) (n = 17) percentage of Tfh cells (b), CXCR3+ Tfh cells (c), CXCR3− Tfh cells (d), and the ratio of CXCR3+/CXCR3− Tfh cells (e) and recovered COVID-19 individuals (n = 26). In b-e, data presented as median, interquartile range (25%-75%). Mann-Whitney U test was used to compare the difference between two groups, and p < 0.05 was considered to be a two-tailed significant difference.

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