Extended Data Fig. 7: Degradation of SLFN5 by other DNA viruses.
a, HFF cells were mock infected or infected with HSV-2 at an MOI of 3, and MG132 (5 µM) was added at 2 h after virus inoculation as indicated. Total cell lysates were prepared at indicated time points and examined by immunoblot analysis with indicated antibodies. ICP8 serves as an infection control. b, HFF cells were mock infected or infected with HCMV (TB40/E) at an MOI of 3 and total cell lysates were prepared at indicated time points and examined by immunoblot analysis with indicated antibodies. UL44 serves as an infection control. Circle marks cross-reacting viral protein. c, A549 cells were mock infected or infected with adenovirus (Ad5) at an MOI of 20 and MG132 (5 µM) was added at 2 h after virus inoculation, as indicated. Cell lysates were prepared at the indicated time points and examined by immunoblot analysis with indicated antibodies. RAD50 served as a degradation control and DBP as a control for infection. Immunoblots show representative data from n = 3 biologically independent experiments.
