Extended Data Fig. 8: Restriction of other DNA viruses by SLFN5.
HeLa cells were mock infected or infected with other DNA viruses as indicated. a, Immunoblot analysis demonstrating increased HSV-2 protein levels in SLFN5-depleted cells. b, HCMV proteins are slightly increased in SLFN5-depleted cells at immediate-early time point (24 hpi), but decreased at late times (72 hpi) as compared to control cells. The ISG15 protein was increased in the absence of SLFN5 and further increased upon HCMV infection. c, SLFN5 does not affect adenovirus protein levels. d, SLFN5 knockdown results in higher level of ISG15 expression in HFF and HeLa cells. Immunoblots show representative data from n = 3 biologically independent experiments.
