Extended Data Fig. 9: Effects of SLFN5 on HCMV infection.
a, b, HCMV mRNA (UL123, an immediate-early gene and UL94, a late gene) expression from shRNA-transduced HFF cells infected with HCMV at an MOI of 3 and harvested at 12 h (a) and 72 h (b) after infection. Quantification of viral transcripts was carried out by RT-qPCR. Viral DNA replication yields from shRNA-transduced HFF cells infected with ΔRING virus at an MOI of 0.1 and harvested at 24 h after infection. Quantification of viral DNA was carried out by qPCR. c, Progeny production of HCMV was monitored by infectious center assays using anti-IE1 antibody. n = 3 biologically independent experiments. Data are the mean ± SD. Comparisons between groups were performed using the two-tailed unpaired Student’s t-test. *, p < 0.05, **, p < 0.005.
