Extended Data Fig. 8: The NLRP3 T659A mutant inhibit the IL-1β maturation triggered by SopE and DNT.
a, HEK293T cells were transfected with plasmids encoding NLRP3 inflammasome components (ASC-GFP, mCaspase-1) and pro-IL-1β-Flag and either myc-NLRP3 (WT) or myc-NLRP3 T659A together with SopE-HA and treated with MG132 (10 μM) to block SopE degradation. Supernatants and cell lysates were analyzed by immunoblot. b, NLRP3 knock-out iBMDMs reconstituted either with NLRP3 or NLRP3 T659A were treated with vehicle or LPS (100 ng/mL) and DNT (1 μg/mL) for 8 h. The numbers on the side of the immunoblots indicate molecular weight (kDa). Experiments were repeated at least three times, and representative data are shown.
