Extended Data Fig. 10: E. coli CNF1− clearing is not affected by Pak1 or NLRP3 inhibition and E. coli CNF1+ clearing does not rely on GSDMD.
a-d, Wild-type or knock-out mice were infected intravenously with isogenic CNF1-deleted E. coli (E. coli CNF1−) or CNF1 expressing E. coli (E. coli CNF1+). a, Wild-type mice were injected intraperitoneally with 10 mg/kg AZ13711265 or 50 mg/kg MCC950 or vehicle once a day and were infected intravenously with isogenic CNF1-deleted E. coli (E. coli CNF1−) prior to the collection of peripheral blood at 4 h, 24 h and 48 h for measurement of bacteraemia (n = 5 mice per group). b, Wild-type or NLRP3 knock-out C57BL/6 J mice were infected intravenously with CNF1 expressing E. coli (E. coli CNF1+) prior to the collection of peripheral blood at 4 h, 24 h, 48 h, 72 h and 96 h for measurement of bacteraemia (n = 6 per group). c, Wild-type (n = 6 mice) or NLRP3 knock-out C57BL/6 J mice (n = 4 mice) were infected intravenously with isogenic CNF1-deleted E. coli (E. coli CNF1−) prior to the collection of peripheral blood at 4 h, 24 h and 48 h for measurement of bacteraemia (n = 6 per group). d, Wild-type (n = 6 mice) or GSDMD knock-out C57BL/6 J mice (n = 6 mice) were infected intravenously with E. coli CNF1+ prior to the collection of peripheral blood at 4 h, 24 h and 48 h for measurement of bacteraemia. Experiments were repeated two times and representative data are shown. Data are expressed as the geometric mean ± 95 CI.
