Fig. 4: Accumulation of myeloid cells in BAL of infected rhesus macaques.
From: Responses to acute infection with SARS-CoV-2 in the lungs of rhesus macaques, baboons and marmosets
a–h, Flow cytometry analysis of BAL IMs (a,e), AMs (b,f), neutrophils (c,g) and pDCs (d,h). For a–d, data are combined for age (n = 12). For e–h, data are split by age (n = 6). Statistical analysis was performed using one-way (a–d) and two-way (e–h) repeated-measures ANOVA with Geisser–Greenhouse correction for sphericity and Tukey post hoc correction for multiple testing (GraphPad Prism 8). The shapes indicate old (triangles) and young (diamonds) animals. The different colours represent individual animals (Supplementary Table 1). n = 12. i,j, Spearman’s rank correlation between the cellular fraction and log10-transformed vRNA copy number in BAL was calculated (i) and the corresponding values for the Spearman’s rank correlation coefficient (j, left) and P values (j, right) are shown. Multilabel confocal immunofluorescence microscopy of formalin-fixed paraffin-embedded lung sections from rhesus macaques infected with SARS-CoV-2 with a high viral titre at 3 d.p.i., stained with DAPI (blue) (k–v); SARS-CoV-2 spike-specific antibodies (k–s) (turquoise); Ki67 (magenta) and neutrophil marker CD66abce (yellow) (k–m); pan-macrophage marker CD68 (magenta) (n–p); HLA-DR (magenta) and pDC marker CD123 (yellow) (q–s); SARS-CoV-2 nucleocapsid-protein-specific antibodies (turquoise), pan-cytokeratin (magenta) and thyroid transcription factor-1 (yellow) (t–v). For k–v, scale bars, 20 μm (×63 magnification; k, n, q and t), 50 μm (×20 magnification; l, o, r and u) and 100 μm (×10 magnification; m, p, s and v). For a–h, P values are indicated above the plots; data are mean ± s.e.m.
