Extended Data Fig. 5: L. monocytogenes-induced LAP in McuΔmye macrophages depends on NOX2. | Nature Microbiology

Extended Data Fig. 5: L. monocytogenes-induced LAP in McuΔmye macrophages depends on NOX2.

From: RETRACTED ARTICLE: Listeria monocytogenes upregulates mitochondrial calcium signalling to inhibit LC3-associated phagocytosis as a survival strategy

Extended Data Fig. 5

a, Confocal imaging of DQ-ovalbumin in Mcu fl/fl, Mcu ∆mye, Cybb−/− and Mcu Δmye Cybb −/− BMMs challenged with L. monocytogenes (MOI, 10) for 2 h Scale bar, 10 μm. b-c, Confocal imaging (b) and quantification (c) of the colocalization of LysoTracker (red) and L. monocytogenes (green) in Mcu fl/fl, Mcu ∆mye, Cybb−/− and Mcu Δmye Cybb −/− BMMs challenged with GFP-L. monocytogenes for indicated periods. Scale bar, 2 μm. The averages of n = 3 biologically independent samples are shown. The error bars represent the SEM. Statistical significance was determined using t test (and nonparametric tests).

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