Extended Data Fig. 10: Importance of residues 1–7, 10, 11, 12, 13 and 16 of SpeFL.
From: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
a, Toeprinting assay8,9 to monitor the translation of (a–c) wild-type speFL and (a) speFLΔ1–7, (b) speFL-R12A-R13A (A12A13) and speFL-R12K-R13K (K12K13), and (c) speFL-H10A, speFL-I11A and speFL-H16A, in the absence (–) or presence ( + ) of 10 mM ornithine, release factors (RF1,2,3) or 90 μM puromycin. Arrows indicate ribosomes stalled with the indicated amino acid in the P-site (Ala33 – open triangle; Arg34 – filled triangle). This experiment was repeated three times independently with similar results.
