Extended Data Fig. 2: The amino acid sequence of SpeFL is important for ornithine-dependent translational arrest.
From: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
Toeprinting assay8,9 to monitor the translation of wild type (WT) and double frameshifted (speFLfs) speFL in the presence ( + ) or absence (–) of 10 mM ornithine, release factors (RF1,2,3) or 90 μM puromycin. Arrows indicate ribosomes stalled with the codon for the indicated amino acid in the P-site (Ala33 – open triangle; Arg34 – filled triangle). This experiment was repeated three times independently with similar results.
