Extended Data Fig. 5: Flowchart of cryo-EM data processing for the SpeFL-ESRF and SpeFL-DLS datasets.
From: Ornithine capture by a translating ribosome controls bacterial polyamine synthesis
Steps where Relion 2.140 and Relion 3.041 were used are shown in purple and green, respectively. The step where Cryosparc 0.642 was used is indicated with an asterisk. Note the increase in resolution when using Relion 3.0 compared to Relion 2.1. This increase was also matched by the quality of the resulting cryo-EM density. Both structures could be refined to an overall resolution of 2.7 Å using a Fourier shell correlation (FSC) cutoff of 0.143.
