Extended Data Fig. 10: Control experiments and flow cytometry methods for DNBS colitis.
From: Spatially distinct physiology of Bacteroides fragilis within the proximal colon of gnotobiotic mice
a, Quantitative RT–qPCR (∆∆Ct method normalized to gyrB) for PSA flippase (BF1369) in lumen, mucus and tissue samples (mean and standard error, n = 4 animals). Fold-change between sample sites was quantified within each mouse individually. b, Mice mono-colonized with indicated strains of B. fragilis for one month were treated with 50% ethanol, the vehicle control for DNBS colitis induction. Mice were weighed every 24 hours, graphed as a percentage of their weight at day 0 (Tukey 2-way ANOVA, n = 5, 4, 4). c, 72 hours after treatment the mice were sacrificed and the length of the colon was measured from rectum to the cecal junction (Tukey 2-way ANOVA, n = 5, 4, 4) d, Example live cell gating for flow cytometry in Extended Data 9f and 9 g (representative from two independent experiments with similar results). e, Example flow plots (1 from each group) for assessing the proportion of IL-10 and IL-17 positive regulatory T cells, as quantified in Extended Data 9f and g (representative from two independent experiments with similar results, mean and standard error in graphs, * p < 0.05).
