Extended Data Fig. 1: Effects and production of volatiles from Streptomyces coelicolor.
Captures of insects (a) and arachnids (b) on the ground of a field site using sticky traps baited with Streptomyces coelicolor cultured on agar medium, compared to control traps that were kept without bait or held a fresh, non-inoculated plate of agar culture medium (n=19). The boxplots show the medians, lower- and upper quartiles as well as the span of data points that are within 1.5 times the interquartile range. Electroantennographic responses (c) towards 2-MIB or geosmin puffed into an air stream passing over antennae of the springtails Folsomia candida. The average responses to all tested stimuli (n=8 per stimulus) significantly differed from the antennal response to charcoal filtered air. Statistical difference is labelled by different letters above the columns; error bars show the standard errors (X25, 48 =629.54, P<0.001). GC-MS chromatograms (d) of S. coelicolor wild type and two biosynthetic mutants show that geosmin or geosmin and 2-MIB, respectively, are missing from the geoA and geoA mibAB mutants (strains J3003 and J2192, respectively). The headspace collection for the specific strains was repeated three times with similar results.
