Extended Data Fig. 5: Effects of developmental regulatory genes bldM and whiH on production of geosmin and 2-methylisoborneol (2-MIB) in Streptomyces venezuelae.
(a) Gas chromatograms analysed for the presence of 2-MIB, geosmin and germacrene D in Streptomyces venezuelae headspace sampled from wild-type, whiH, bldM mutants or a system control. The chromatograms and the quantification of Streptomyces metabolites (b; n=3; error bars give the standard deviation of the mean) illustrate that in comparison to wild-type, geosmin and germacrene D were reduced in the whiH mutant. None of the three Streptomyces metabolites was detected (n.d.) in the bldM mutant or the control. C12 –C14 hydrocarbons were used for calculation of retention indices. Octyl acetate was introduced with the internal standard heptyl acetate (not shown), which was used for quantification.
