Extended Data Fig. 1: Cryo-EM reconstruction. | Nature Microbiology

Extended Data Fig. 1: Cryo-EM reconstruction.

From: Structure of severe fever with thrombocytopenia syndrome virus L protein elucidates the mechanisms of viral transcription initiation

Extended Data Fig. 1

a, Raw image of SFTSV-L particles in vitreous ice recorded at defocus values of -1.5 to -2.5 μm. Scale bar, 100 nm. b, Power spectrum of the image shown in (a), with plot of the rotationally averaged intensity versus resolution. White circle indicates the spatial frequency corresponding to 3.0 Å resolution. c, Representative class averages. The edge of each square is 238 Å. d, Overview of SFTSV-L reconstruction is shown in the left panel. The close-up view of a representative region in the RdRp region is shown in the right panel. Density is shown as gray mesh; the polypeptide of the refined model is displayed as colored sticks (carbons, green; nitrogen, blue; oxygen, red). e, Fourier shell correlation (FSC) of the final 3D reconstruction following gold standard refinement. FSC curves are plotted before (red) and after (green) masking in addition to post-correction (blue), accounting for the effect of the mask using phase randomization. f, Local resolution estimation was performed with Relion 2.11. g, The 3DFSC sphericity was analyzed with 3DFSC2. h, The data processing scheme used to obtain the final map.

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