Extended Data Fig. 10: PRC1 effects on latency III expression and EBV H2Ak119 monoubiquitylation.
From: DNA methylation enzymes and PRC1 restrict B-cell Epstein–Barr virus oncoprotein expression
a, Immunoblot analysis of WCL from GM12878 LCLs that expressed GFP versus RING1 cDNAs or from MUTU I or III as controls. b, FACS analysis of PM abundances of LMP1/NF-kB targets Fas/CD95 (left) and ICAM-1 (right) in GM12878 LCL expressing GFP (blue) versus RING1 (red). c, ChIP analysis of chromatin from MUTU 1 (black bar) or MUTU III (blue bar) using control IgG versus anti-H2A lysine 119 monoubiquitin (H2AK119Ub) followed by qPCR with primers specific for LMP1p, LMP2p or Cp. Mean ± SEM from n = 3 biologically independent replicates are shown, p-values were calculated by unpaired two-sided student’s t-test with equal variance assumption. d, Schematic model of PRC1 complex-mediated ubiquitination of chromatin. a and b are representative of n = 3 biologically independent replicates.
